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M7443

Anti-MeCP2 antibody, Mouse monoclonal

clone Men-8, purified from hybridoma cell culture

Synonym(s):

Anti-AUTSX3, Anti-MRX16, Anti-MRX79, Anti-MRXS13, Anti-MRXSL, Anti-PPMX, Anti-RS, Anti-RTS, Anti-RTT

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD02686372
Conjugate:
unconjugated
Clone:
Men-8, monoclonal
Application:
ARR, ELISA (i), ICC, WB
Citations:
19
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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

Men-8, monoclonal

form

buffered aqueous solution

mol wt

antigen ~75 kDa

species reactivity

mouse, human, rat, monkey

packaging

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/test using nuclear extract of cultured human acute T cell leukemia Jurkat cells or MCF7 cells

isotype

IgG1

UniProt accession no.

P51608

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MECP2(4204)
mouse ... Mecp2(17257)
rat ... Mecp2(29386)

General description

Methyl-CpG binding protein 2 (MeCP2) possesses a methyl-CpG-binding domain (MBD) and a transcriptional repression domain.
Monoclonal Anti-MeCP2 (mouse IgG1 isotype) is derived from the Men-8 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the N-terminus of human MeCP2.

Immunogen

synthetic peptide corresponding to the N-terminus (amino acids 15-30) of human MeCP2.

Application

Monoclonal Anti-MeCP2 antibody has been used:
  • in western blotting
  • in immunoblotting
  • in flow cytometry
  • in immunohistofluorescence
  • in immunocytochemistry

Biochem/physiol Actions

MeCP2 (methyl-CpG binding protein 2) silences transcription by recruiting the histone deacetylase (HDAC) repressive machinery via recruitment of the Sin 3A corepressor, thus removing acetyl groups from histones and consequently, silencing genes.
Methyl-CpG binding protein 2 (MeCP2) has been shown be crucial for brain development. It part of the methyl-CpG-binding proteins, which are involved in repression of gene expression by binding to methylated DNA. Mutations in the gene encoding MeCP2 have been associated with Rett syndrome (RTT).

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
常规特殊物品
低风险生物材料

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Certificates of Analysis (COA)

Lot/Batch Number
22140521
019M4793V
116M4847V
125M4851
114M4808

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Related Content

Paul D Ross et al.
Human molecular genetics, 25(20), 4389-4404 (2017-02-09)
Rett syndrome (RTT) is a severe genetic disorder resulting from mutations in the X-linked MECP2 gene. MeCP2 protein is highly expressed in the nervous system and deficiency in the mouse central nervous system alone recapitulates many features of the disorder.
Radically truncated MeCP2 rescues Rett syndrome-like neurological defects
Tillotson R, et al.
Nature, 550(7676), 398-398 (2017)
Abhishek Banerjee et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(46), E7287-E7296 (2016-11-03)
Rett syndrome (RTT) arises from loss-of-function mutations in methyl-CpG binding protein 2 gene (Mecp2), but fundamental aspects of its physiological mechanisms are unresolved. Here, by whole-cell recording of synaptic responses in MeCP2 mutant mice in vivo, we show that visually
View All Related Papers

Global Trade Item Number

SKUGTIN
M7443-200UL04061837794827
M7443-25UL04061838802743