MAK003
Branched Chain Amino Acid Kit
sufficient for 100 colorimetric tests
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; gastrointestinal diseases; neurological disorders
storage temp.
−20°C
General description
The branched-chain amino acids (BCAA), leucine, isoleucine, and valine, are the most common essential amino acids in proteins. The BCAA may function in both energy production and as nutrient signals. Dietary supplementation with BCAA may aid in the treatment of various disorders such as Amyotrophic Lateral Sclerosis (ALS), latent and chronic hepatic encephalopathies, and to prevent muscle atrophy in cancer patients. BCAA may increase the rate of protein synthesis and sensitize cells to insulin and insulin-like growth factor.
Application
Branched Chain Amino Acid Kit has been used to determine the concentration of hepatic branched-chain amino acids, in a study assessing the role of CREB regulated transcription coactivator 2 (CRTC2) in hepatic lipid homeostasis and obesity in mice.
Suitable for measuring Branched Chain Amino Acids (BCAA′s) in a variety of biological samples.
Biochem/physiol Actions
In this assay, BCAA concentration is determined using a coupled enzyme reaction, which results in a colorimetric (450 nm) product, proportional to the BCAA present. The BCAA Assay Kit provides a simple convenient means of measuring the BCAAs in a variety of biological samples.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
Regulatory Information
常规特殊物品
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Articles
Assay protocol for the colorimetric detection of Branched Chain Amino Acids (BCAA) in biological samples using the alanine assay kit.
Related Content
Protein substitution to produce a processed cheese with high branched-chain amino acids of medical and genetic importance.
El-Shazly H A M, et al.
The Egyptian journal of medical human genetics., 11(2), 121-133 (2010)
The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1.
Han J, et al.
Nature, 524(7564), 243-243 (2015)
Multifaceted role of insulin-like growth factors and mammalian target of rapamycin in skeletal muscle.
Frost R A and Charles H L
Endocrinol. Metab. Clin., 41(2), 297-322 (2012)