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Merck
CN

MAK046

Lipase Activity Assay Kit

sufficient for 100 colorimetric tests (with glycerol standard)

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
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usage

sufficient for 100 colorimetric tests (with glycerol standard)

detection method

colorimetric

relevant disease(s)

pulmonary disorders; gastrointestinal diseases; cancer

storage temp.

−20°C

General description

The lipase family of enzymes catalyze the cleavage of the ester bonds of lipids. In mammals, this family includes many critical members including pancreatic, hepatic, endothelial, and lipoprotein lipase. Lipases, such as pancreatic lipase, are critical for the metabolism of lipids. Lipases also play key roles in processes such as cell signaling and inflammation. Measurements of lipase activity are commonly used to screen for pancreatic injury or disease, and to monitor diseases such as cystic fibrosis, celiac disease, and Crohn′s disease.

Application

Lipase Activity Assay Kit has been used to determine the lipase activity in samples.
Suitable for the detection of lipase activity in biological samples including tissue, cells and serum

Biochem/physiol Actions

The Lipase Activity Assay kit provides a simple and direct procedure for measuring lipase activity in a variety of samples. Lipase activity is determined using a coupled enzyme reaction, which results in a colorimetric (570 nm) product proportional to the enzymatic activity present. One unit of Lipase is the amount of enzyme that will generate 1.0 μmole of glycerol from triglycerides per minute at 37 °C.

Features and Benefits

Compatible with high-throughput handling systems.


signalword

Danger

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

wgk

WGK 3

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Regulatory Information

常规特殊物品

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Enhanced absorption of long-chain polyunsaturated fatty acids following consumption of functional milk formula, pre-digested with immobilized lipase ex vivo, in an exocrine pancreatic insufficient (EPI) pig model.
Goncharova K, et al.
Journal of functional foods, 34, 422-430 (2017)
Effect of phenolic hydroxyl groups on inhibitory activities of phenylpropanoid glycosides against lipase.
Wu X, et al.
Journal of functional foods, 38, 510-518 (2017)
Amélie de Vallée et al.
Frontiers in microbiology, 10, 2829-2829 (2019-12-24)
The gray mold fungus Botrytis cinerea is a necrotrophic pathogen able to infect hundreds of host plants, including high-value crops such as grapevine, strawberry and tomato. In order to decipher its infectious strategy, a library of 2,144 mutants was generated



Global Trade Item Number

SKUGTIN
MAK046-1KT04061834064923