Lipase Assay Kit

Lipase catalyzes the hydrolysis of ester bonds on the glycerol backbone of a lipid substrate. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglycerides to monoglycerides and free fatty acids. Human pancreatic lipase and its related protein 2 are the main lipases secreted by the pancreas. In acute pancreatitis, lipase levels can rise 5 to 10-fold within 24 to 48 hours. Increased activities have also been associated with pancreatic duct obstruction, pancreatic cancer, kidney disease, salivary gland inflammation, bowel obstruction, and other pancreatic diseases. Decreased levels may indicate permanent damage to lipase-producing cells in the pancreas.

The kit is suitable for detecting lipase activity in serum, plasma, saliva, urine and other biological samples.

The Lipase Assay Kit is based on an improved Dimercaptopropanol tributyrate (BALB) method, in which SH groups formed from lipase cleavage of BALB react with 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) to form a yellow-colored product. The color intensity, measured at 412 nm, is proportionate to the enzyme activity in the sample.