Kidney PTEC MDR1 KnockoutCells [MDR1 knock out (-/-)]

Sigma modified human primary renal proximal tubule epithelial cells (RPTEC) with ZFNs to effectively create novel human RPTEC cell lines that can be utilized for investigating kidney toxicology (nephrotoxicity), transporters and various other renal studies. Sigma′s RPTEC′s exhibit normal epithelial morphology and expression of Renal Proximal Tubule (PT) specific markers such as, CD13 and α-GST. Sigma′s RPTEC′s maintain typical PT functions including albumin reabsorption, γ-glutamyl transpeptidase (GGT) activity, and respond to parathyroid hormone. The cells respond to several known human nephrotoxicants similar to human primary cells and demonstrate robust expression and activity of key kidney uptake and efflux transporters incuding OCT2, OATP4C1, OAT1, OCTN1, MRP2, MRP4, P-gp, MATE1, MATE2-K, PEPT1, and PEPT2.

Human RPTEC SA7K control cell line and transporter KO models have been validated in several standard and 3D assays for measuring nephrotoxicity and transporter assays. RPTEC SA7K control cells can be used to measure toxicity of compounds using dose-response curves and viability such as MTT based in vitro Toxicology in both 2D and 3D culture.

RPTEC SA7K control cells can also be used to measure compound uptake/efflux by transporters using inhibitors. Precision editing in RPTEC SA7K transporter KO cells provides a more predictive platform for compound uptake/efflux by transporters without the need for non-specific inhibitors.

RPTEC SA7K cells have been validated for use on the Mimetas 3-lane OrganoPlate^® in both toxicity and barrier integrity assays (( Z742750).

RPTEC SA7K cells can also be used on the AIM Biotech 3D cell culture chip in toxicity and permeability assays ( DAX01).

Sigma′s human RPTEC control SA7K clone cells and knockout (KO) cells exhibit normal renal proximal tubule characteristics, functional renal uptake and efflux transporters and are sensitive to human nephrotoxicants making them ideal for studying drug-transporter interactions and early detection of renal toxicants. The SA7K clone should be used as a control for Sigma′s human RPTEC KO cells

• Sigma′s human RPTEC MDR1 (P-gp) Knockout (KO) allows investigations of drug transporter interactions involving P-gp in the kidney.

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

ADME/Tox cell lines license. These products are covered by the “Consumable Vial” License as described in Exhibit 1.

These products are covered by the ADME/Tox Cell Lines License as described in Exhibit 1, in the technical bulletin.

OrganoPlate is a registered trademark of MIMETAS B.V.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.