MTOXRCSUP
RPTEC Complete Supplement
sterile-filtered, suitable for cell culture, add to 500 ML of basal medium
Synonym(s):
Basal media supplement for huRPTEC
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.81
UNSPSC Code:
12352207
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
storage temp.
−20°C
General description
Sigma has developed a variety of specialized media which are intended to provide optimal performance when utilized with specific cell lines. This product consists of 30 mL of RPTEC complete media supplement which is added to basal media and other components listed below to produce 500 mL of complete media needed for optimal growth, maintenance, and culture of Sigma′s Human Renal Proximal Tubule Epithelial Cells (RPTEC). The final RPTEC complete media was developed specifically for use with Sigma′s human RPTEC control SA7K Clone cells and various knockouts (KO).
Application
RPTEC Complete Supplement has been used in SA7K cell line culture for cell characterization.
Preparation Note
Prepare Complete Medium by supplementing 500 mL of MEMα (M4526) with 6.25 mL of L-Glutamine (G7513), 30 mL of RPTEC Complete Supplement (MTOXRCSUP), 0.3 mL of Gentamicin solution (G1397) and 0.03 mL of Amphotericin B solution (A2942). Complete Medium can be stored at 2–8 °C for up to 1 month. This medium is formulated for use with a 5% CO2 in air atmosphere.
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
高风险级别生物产品--人源产品
低风险生物材料
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
AIM Biotech's modular platform enables simple to complex 3D cell culture for various research needs.
Human renal proximal tubule epithelial cells (RPTECs) are commonly used to predict human renal drug permeability and to investigate drug efflux. We have generated transporter knockout (KO) cell lines using CompoZr® Zinc Finger Nuclease (ZFN) technology in a proprietary renal proximal tubule epithelial cell line.
Related Content
Development and application of human renal proximal tubule epithelial cells for assessment of compound toxicity.
Li S, et al.
Current Chemical Genomics and Translational Medicine, 11, 19-19 (2017)
Marianne K Vormann et al.
The AAPS journal, 20(5), 90-90 (2018-08-16)
Proximal tubules in the kidney play a crucial role in reabsorbing and eliminating substrates from the body into the urine, leading to high local concentrations of xenobiotics. This makes the proximal tubule a major target for drug toxicity that needs
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MTOXRCSUP-30ML | 04061834076339 |