N3643
NZCYM Broth
Powder microbial growth medium
Synonym(s):
Broth medium
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About This Item
NACRES:
NA.85
UNSPSC Code:
41106200
grade
Molecular Biology
Quality Level
sterility
non-sterile
form
powder
composition
Casamino Acids, 1 g/L , MgSO4, 0.98 g/L , NaCL, 5 g/L , Tryptone, 10 g/L , Yeast extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(2.2% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
NZCYM is a microbial growth medium used for the cultivation of E. coli and lambda bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Application
NZCYM Broth has been used for non-selective cultivation of lambda, MS2, T7, CLP-P3, Phi29, and Goe1 bacteriophages, and E. coli strains for cloning, DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.
Features and Benefits
NZCYM powder provides:
- Standard formulation
- Convenient package sizes
- A budget-friendly alternative to liquid
Preparation Note
Stir to suspend 22 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
监管及禁止进口产品
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Protocols
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
General protocols for growth of competent cells in microbial medium.
Shane R Wilkinson et al.
The Journal of biological chemistry, 277(19), 17062-17071 (2002-02-14)
Trypanosoma cruzi glutathione-dependent peroxidase I (TcGPXI) can reduce fatty acid, phospholipid, and short chain organic hydroperoxides utilizing a novel redox cycle in which enzyme activity is linked to the reduction of trypanothione, a parasite-specific thiol, by glutathione. Here we show
Bioreagents for Cloning
Sigma data, 2-2 (2011)
Chris Coward et al.
Applied and environmental microbiology, 72(7), 4638-4647 (2006-07-06)
This study characterizes the interaction between Campylobacter jejuni and the 16 phages used in the United Kingdom typing scheme by screening spontaneous mutants of the phage-type strains and transposon mutants of the sequenced strain NCTC 11168. We show that the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| N3643-1KG | 04061826764213 |
| N3643-250G | 04061833655030 |