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N6514

Neuraminidase from Vibrio cholerae

Name: Neuraminidase from <I>Vibrio cholerae</I>
Brand: SIGMA

Type II, buffered aqueous solution, 8-24 units/mg protein (Lowry, using NAN-lactose)

Synonym(s):

Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase

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About This Item

CAS Number:

9001-67-6

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-624-6

MDL number:

MFCD00131711

EC Number:

3.2.1.18 (BRENDA, IUBMB)

Specific activity:

8-24 units/mg protein (Lowry, using NAN-lactose)

Biological source:

Vibrio cholerae

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Properties

Quality Level

200

biological source

Vibrio cholerae

type

Type II

form

buffered aqueous solution

specific activity

8-24 units/mg protein (Lowry, using NAN-lactose)

foreign activity

Protease and NAN-aldolase, present

storage temp.

2-8°C

Description

General description

Neuraminidase enzymes are hydrolase enzymes that promote influenza virus release from infected cells and facilitate virus spread.

Calorimetry studies show that the two lectin-like domains flanking the central catalytic domain of Neuraminidase serve as the recognition and binding sites for sialic acid-containing substrates.

Application

Neuraminidase from Vibrio cholera has been used in a study to describe a five-step purification method. It has also been used in a study to investigate modification of leukemia L1210 tumor cells.

Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.

Physical form

Solution in 50 mM sodium acetate, pH 5.5, containing 0.15 M sodium chloride and 4 mM calcium chloride, 0.2 μm filtered..

Preparation Note

A further purification by affinity chromatography of our Type III.

Analysis Note

Preservative free.

Other Notes

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.