p-Nitrophenyl Phosphate Liquid Substrate System

Name: <I>p</I>-Nitrophenyl Phosphate Liquid Substrate System
Brand: SIGMA

alkaline phosphatase substrate, liquid

Synonym(s):

4-Nitrophenyl phosphate disodium salt solution, pNPP

Select a Size

Change View

About This Item

Linear Formula:

O₂NC₆H₄OP(O)(ONa)₂

CAS Number:

4264-83-9

Molecular Weight:

263.05

UNSPSC Code:

12352204

PubChem Substance ID:

24897835

NACRES:

NA.83

MDL number:

MFCD00007319

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

p-Nitrophenyl Phosphate Liquid Substrate System, liquid

form

liquid

Quality Level

200

shipped in

wet ice

storage temp.

−20°C

SMILES string

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

InChI key

VIYFPAMJCJLZKD-UHFFFAOYSA-L

Description

General description

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

Application

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.

Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.