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P0609

Pepsin−Agarose from porcine gastric mucosa

Name: Pepsin−Agarose from porcine gastric mucosa
Brand: SIGMA

lyophilized powder, ≥30 units/mg dry solid

Synonym(s):

Pepsin A

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.4.23.1 (BRENDA, IUBMB)

MDL number:

MFCD00081840

Specific activity:

≥30 units/mg dry solid

Biological source:

Porcine gastric mucosa

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Properties

biological source

Porcine gastric mucosa

Quality Level

200

form

lyophilized powder

specific activity

≥30 units/mg dry solid

mol wt

35 kDa

matrix

4% cross-linked beaded agarose

UniProt accession no.

P00791

storage temp.

−20°C

Gene Information

pig ... LOC396892(396892)

Description

General description

Pepsin is an enzyme found in gastric secretion.

Application

Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin.

Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also been used for the preparation of F(ab′)2 fragments from rabbit IgG.

Used to produce F(ab′)₂ fragments of antibodies.¹

Biochem/physiol Actions

Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. pH optimum 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH > 6.

Physical form

Lyophilized powder stabilized with lactose

Preparation Note

One gram solid swells to 5-10ml packed gel.

Other Notes

One unit will produce a ΔA₂₈₀ of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P304 + P340 - P342 + P311 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Information

低风险生物材料

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Protocols

胃蛋白酶的酶法测定（3.4.23.1）

本方法可用于以血红蛋白为底物的胃蛋白酶活性测定。这是一种分光光度速率终止测定法。

Enzymatic Assay of Pepsin (3.4.23.1)

This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.

Articles

Antibody Fragmentation with Pepsin Digestion

Antibody fragmentation with our pepsin digestion protocol for IgG antibody fragmentation and preparation of F(ab’).

Global Trade Item Number

SKU	GTIN
P0609-50KU	04061834323068
P0609-10KU	04061832700977
P0609-25KU	04061832700984