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P1874

Anti-Huntingtin Disease (HD/HTT) Antibody

mouse monoclonal, 3B5H10

Synonym(s):

Anti-Huntingtin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD01322176
Conjugate:
unconjugated
Clone:
3B5H10, monoclonal
Application:
ICC, IP, WB
Citations:
21
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Product Name

Anti-Polyglutamines antibody, Mouse monoclonal, ~2 mg/mL, clone 3B5H10, purified from hybridoma cell culture

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

3B5H10, monoclonal

form

buffered aqueous solution

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 1-2 μg/mL using extract of HEK-293T cells transfected with an N-terminal 171 amino acid fragment of human Huntingtin with a 68 glutamine stretch

isotype

IgG1

UniProt accession no.

P42858

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HTT(3064)

General description

Abnormal stretch of triplet CAG (or CAA) codon produces a homomeric repeat of glutamine residues beyond the critical threshold and will lead to neurodegenerative disease. Monoclonal mouse anti-polyglutamine antibody can be used to detect poly Q protein expression and in western blot. Monoclonal anti-polyglutamines antibody reacts specifically to homomeric polyglutamines independent of the species.

Immunogen

GST-human Huntingtin (N-terminal fragment of 171 amino acids containing 65Q).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal anti-polyglutamines antibody may be used for ELISA, immunoblot and slot blot. It can also be used in immunoprecipitation and immunocytochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

This product is for in vitro use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California. This product is sold under license to U.S. Patent No. 6,291,652, owned by the Regents of the University of California.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
常规特殊物品
低风险生物材料

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Certificates of Analysis (COA)

Lot/Batch Number
054M4861
099M4794V
108M4866V
047M4820V
052M4843

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Elizabeth Brooks et al.
Methods in molecular biology (Clifton, N.J.), 277, 103-128 (2004-06-18)
Expansion of a homomeric stretch of glutamine residues beyond a critical threshold can produce neurodegenerative disease. This observation led to the idea that abnormal polyglutamine stretches can alter protein structure in ways that contribute to disease. Because they are prone
Jennifer L Wacker et al.
Nature structural & molecular biology, 11(12), 1215-1222 (2004-11-16)
Protein conformational changes that result in misfolding, aggregation and amyloid fibril formation are a common feature of many neurodegenerative disorders. Studies with beta-amyloid (Abeta), alpha-synuclein and other amyloid-forming proteins indicate that the assembly of misfolded protein conformers into fibrils is
Kirupa Sathasivam et al.
Human molecular genetics, 19(1), 65-78 (2009-10-15)
Huntington's disease (HD) is a late-onset neurodegenerative disorder that is characterized neuropathologically by the presence of neuropil aggregates and nuclear inclusions. However, the profile of aggregate structures that are present in the brains of HD patients or of HD mouse
View All Related Papers

Global Trade Item Number

SKUGTIN
P1874-100UL04061838164438
P1874-200UL04061838106742
P1874-25UL04061838805720