P2545
Protein A–Agarose
saline suspension
Synonym(s):
Protein A resin
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About This Item
Form:
saline suspension
Biological source:
Staphylococcus aureus
biological source
Staphylococcus aureus
Quality Level
form
saline suspension
extent of labeling
~2 mg per mL
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
≥15 mg/mL binding capacity (human IgG)
storage temp.
2-8°C
General description
Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.
Application
Protein A−Agarose has been used in the immunoprecipitation
- of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
- of prolyl hydroxylase domain 2 N27 cells
- of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells
Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.
Biochem/physiol Actions
Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.
Physical form
Suspension in 0.5 M NaCl containing preservative
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Storage Class
10 - Combustible liquids
wgk
WGK 3
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Regulatory Information
常规特殊物品
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Related Content
EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001
Membrane targeting of palmitoylated Wnt and Hedgehog revealed by chemical probes
Gao X, et al.
Febs Letters, 585(15), 2501-2506 (2011)
D J McCool et al.
The Biochemical journal, 312 ( Pt 1), 125-133 (1995-11-15)
We have shown previously [McCool, Forstner and Forstner (1994) Biochem. J. 302, 111-118] using pulse-chase labelling of mucin with [3H]threonine that LS180 colonic tumour cells synthesize and secrete MUC2 without the addition of secretagogues. Treatment of the LS180 cells with
PTEN-Dependent Stabilization of MTSS1 Inhibits Metastatic Phenotype in Pancreatic Ductal Adenocarcinoma
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| P2545-1ML | 04061834363996 |
| P2545-5ML | 04061834364009 |