P3369
Protamine sulfate
solid, 90.0-110.0% dry basis,≥92% (chromatography), suitable for transduction
Synonym(s):
Salmine sulfate from salmon sperm, Protamine sulfate
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Product Name
Protamine sulfate, meets USP testing specifications
agency
meets USP testing specifications
Quality Level
assay
≥92% (chromatography), 90.0-110.0% dry basis
form
solid
mol wt
Mr 5000-10000
technique(s)
transduction: suitable
shipped in
ambient
storage temp.
2-8°C
General description
Protamine sulfate is a cationic polypeptide having a molecular weight of 5 kDa, isolated from salmon sperm.
Application
Protamine sulfate has been used in the transduction of
- lentiviruses into human embryonic 293T cells
- shRNA into mesenchymal stem cells/marrow stromal cells (MSC)
- lenti Sca1-OCT4 plasmid into human cord blood hematopoietic progenitor CD34+ cells
Biochem/physiol Actions
Alkaline protamine binds to negatively charged heparin. Use of protamine during cardiovascular surgery sometime triggers anaphylactic reactions.
Protamine facilitates direct transfection of microbial cells, and of human cells using cationic liposomes. Protamine effectively neutralizes the anticoagulation effects of heparin.
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
低风险生物材料
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protamine sulfate down-regulates thrombin generation by inhibiting factor V activation
Ainle FN, et al.
Blood, 114(8), 1658-1665 (2009)
V Diness et al.
Thrombosis and haemostasis, 56(3), 318-322 (1986-12-15)
The neutralization of a low molecular weight heparin (LHN-1) and conventional heparin (CH) by protamine sulfate has been studied in vitro and in vivo. In vitro, the APTT activity of CH was completely neutralized in parallel with the anti-Xa activity.
Serious anaphylactic reactions due to protamine sulfate: a systematic literature review
Nybo M and Madsen JS
Basic and Clinical Pharmacology and Toxicology, 103(2), 192-196 (2008)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P3369-100G | 04061835558292 |
| P3369-10G | 04061835558308 |