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P5368

Phosphate buffered saline

Name: Phosphate buffered saline
Brand: SIGMA

powder, pH 7.4, BioPerformance Certified, for molecular biology

Synonym(s):

PBS

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About This Item

UNSPSC Code:

12161700

eCl@ss:

32129211

NACRES:

NA.25

MDL number:

MFCD00131855

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Properties

Product Name

Phosphate buffered saline, BioPerformance Certified, pH 7.4

grade

BioPerformance Certified, Molecular Biology

form

powder

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

technique(s)

immunofluorescence: suitable

impurities

BioBurden, tested, endotoxin, tested

color

white

pH

7.4

solubility

water: 9.07-10.0 g/L, clear, colorless

cation traces

Fe: ≤10 ppm, heavy metals (as Pb): ≤5 ppm

suitability

suitable for SDS-PAGE, suitable for molecular biology

application(s)

diagnostic assay manufacturing
histology
sample preparation

foreign activity

DNase, RNase, Protease, none detected

SMILES string

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5

InChI key

LOKCTEFSRHRXRJ-UHFFFAOYSA-I

Description

Application

Rapid and zero-cost DNA extraction from soft-bodied insects for routine PCR-based applications.: This study used phosphate buffered saline (PBS) to develop a cost-effective and efficient method for DNA extraction from insects, facilitating routine PCR-based applications in molecular biology (Jangra & Ghosh, 2022).

Injectable amnion hydrogel-mediated delivery of adipose-derived stem cells for osteoarthritis treatment.: PBS was utilized to prepare and deliver adipose-derived stem cells in an injectable hydrogel, showing potential for innovative osteoarthritis treatments (Bhattacharjee et al., 2022).

Rapid and sensitive detection of SARS-CoV-2 infection using quantitative peptide enrichment LC-MS analysis.: This research employed PBS for sample preparation in a novel LC-MS method for detecting SARS-CoV-2, providing a rapid and sensitive diagnostic tool (Hober et al., 2021).

Packaging

Foil Pouch

Preparation Note

Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); pH 7.4, at 25 °C.

Other Notes

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

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Protocols

Biodegradable Nanoparticles

Preparation for biodegradable nanoparticles and their use in transfection protocols .

Articles

Protein Binding Determination - Comparison Study of Techniques & Devices

Comparative analysis of Supel™ BioSPME 96-Pin device with a rapid equilibrium dialysis technique for accuracy of measured values, sample cleanliness, and workflow time in drug protein binding.

Speed, Simplicity and Accuracy of Protein Binding Determination using BioSPME

Learn about Supel™ BioSPME C18 high-throughput devices to determine plasma protein binding and decrease the sample preparation time to less than an hour.

Sample Cleanliness Using BioSPME

Learn about Supel™ BioSPME C18 96-pin tool to prepare a clean sample with minimal matrix effects for an LC-MS/MS analysis from human plasma and its comparison with acetonitrile-based precipitation.

View All Articles

New perspectives to the enterotoxigenic E. coli F4 porcine infection model: Susceptibility genotypes in relation to performance, diarrhoea and bacterial shedding.

Petra J Roubos-van den Hil et al.

Veterinary microbiology, 202, 58-63 (2016-09-27)

Enterotoxigenic E. coli (ETEC), causing post-weaning diarrhoea, is a major problem in weaned piglets. Individual animal responses to ETEC infection show high variability in animal experiments. Two studies were designed to optimize the ETEC F4ac infection model in piglets by

Magnetic Graphene Field-Effect Transistor Biosensor for Single-Strand DNA Detection.

Jinjin Sun et al.

Nanoscale research letters, 14(1), 248-248 (2019-07-26)

Herein, a magnetic graphene field-effect transistor biosensor was prepared through the transfer of a chemical vapor deposition graphene film onto a glass substrate to produce a sensing film and conductive channel. By fixing 1-pyrenebutanoic acid succinimidyl ester onto graphene film

Assessment of the Sensitizing Potential of Proteins in BALB/c Mice: Comparison of Three Protocols of Intraperitoneal Sensitization.

Jesús Gilberto Arámburo-Galvez et al.

Nutrients, 10(7) (2018-07-18)

Most food allergy cases are associated with a limited group of allergens. This could be attributed to an increased ability of some foods to sensitize and trigger allergic reactions. However, there are no validated animal models to evaluate the sensitizing

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Global Trade Item Number

SKU	GTIN
P5368-10PAK	04061835547630
P5368-5X10PAK	04061835547647