P7828
Percoll®
suitable for plant cell culture
Synonym(s):
density gradient medium
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About This Item
sterility
aseptically filled
Quality Level
form
colloidal (Colloidal solution of silica coated with polyvinylpyrrolidone (PVP))
technique(s)
cell culture | plant: suitable
pH
8.9 (20 °C)
application(s)
agriculture
storage temp.
2-8°C
Application
Percoll® has been used in the density gradient centrifugation isolation of intact chloroplasts from green alga, decidual stromal cells and distinct populations of rooster sperm.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used in cell separation and organelle isolation.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.
Percoll is used in plant research for protoplast and organelle (chloroplast, mitochondria) separation.
Legal Information
Percoll is a registered trademark of Cytiva
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Protocols
为了配置Percoll梯度液，必须先用生理盐水或细胞培养基将Percoll调至生理性渗透压。
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
Guangkun Yin et al.
Plant & cell physiology, 50(7), 1305-1318 (2009-06-13)
The development of mitochondria during seed germination is essential for plant growth. However, the developmental process is still poorly understood. Temperature plays a key role in soybean germination, and in this study we characterized the mitochondrial ultrastructure and proteome after
Sperm Collection of Differential Quality Using Density Gradient Centrifugation
Ahammad MU, et al.
Journal of Visualized Experiments, 145(141), e58833-e58833 (2018)
A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtii
Mason CB, et al.
Nature Protocols, 1(5), 2227-2227 (2006)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P7828-100ML | 04061834398219 |
| P7828-1L | 04061834398226 |
| P7828-25ML | 04061834398233 |
| P7828-500ML | 04061834398240 |