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PIC0004

Protease Inhibitor Cocktail

ReadyShield® ready-to-use solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use in purification of Histidine-tagged proteins, Non-freezing solution

Synonym(s):

Protease inhibitor cocktail

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.28
Form:
ready-to-use solution
Solubility:
water: soluble
Storage temp.:
−20°C
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Product Name

ReadyShield® Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, Non-freezing solution

description

Purification of Histidine-Tagged Proteins

Quality Level

100

form

ready-to-use solution

quality

200

packaging

pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)

solubility

water: soluble

storage temp.

−20°C

General description

This particular protease inhibitor cocktail omits chelators (e.g., EDTA), to optimize its use for purification of histidine-tagged proteins.1 It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. The cocktail has been tested on extracts from E. coli and insect cells This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0004 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P8849.

Features and Benefits

  • Does not freeze in storage for quick, convenient use.
  • Tailored for use with Histidine fusion tagged proteins.
  • Suggested as 100 X solution.
  • Reclosable screw-top vials.
  • Can be stored in cooler for approx. 1 month or room temperature for a week.

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 100 ml of lysate from 20 g (wet weight) of E. coli cells or 10 g (wet weight) of baculovirus-infected insect (Spodoptera frugiperda) cells.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

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Exclamation mark
GHS07

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Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Articles

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

Global Trade Item Number

SKUGTIN
PIC0004-5ML04061841255192
PIC0004-1ML04061841255185