Protease and Phosphatase Inhibitor Cocktail

Name: Protease and Phosphatase Inhibitor Cocktail
Brand: SIGMA

DMSO solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidase, serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase

Synonym(s):

Protease and Phosphatase Inhibitor Mix, Protease and phosphatase inhibitor

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About This Item

UNSPSC Code:

12352200

NACRES:

NA.54

Form:

DMSO solution

Solubility:

water: soluble

Storage temp.:

−20°C

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Properties

Product Name

Protease and Phosphatase Inhibitor Cocktail,

form

DMSO solution

Quality Level

200

packaging

pkg of 1 ml, 5 ml ×

solubility

water: soluble

shipped in

wet ice

storage temp.

−20°C

Description

General description

Protease and Phosphatase Inhibitor Cocktail is designed to increase the yield of intact and unmodified proteins in cell extracts. Protease and Phosphatase Inhibitor cocktail mixture inhibits serine, cysteine, and acid proteases, as well as aminopeptidase. It also inhibits serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase. The cocktail has been tested on extracts from various animal tissues such as the human placenta and bovine liver and on extracts of A431, CHO, and U937 cells. Protease and Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO.

Application

Protease and Phosphatase Inhibitor Cocktail has been used as a supplement in radioimmunoprecipitation assay (RIPA) buffer:

for cellular protein extraction
to lyse purified CD4+ T cells
to prepare protein extracts from placental tissues and cells

Protease and Phosphatase Inhibitor Cocktail has been used in protein isolation buffers.

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.

Other Notes

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This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.