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R0883

RPMI-1640 Medium

Name: RPMI-1640 Medium
Brand: SIGMA

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217820

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Properties

Product Name

RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO₃: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used:

to culture PC-3 prostate cell line and Jurkat cells
to maintain a triple-negative MDA-MB-231 and a luminal type MCF7 human Caucasian breast adenocarcinoma cell line
to cultivate circulating tumor cells (CTCs)

Preparation Note

Supplement with 0.3 g/L L-glutamine.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocols

四唑盐法(MTT)细胞活力和增殖检测方案

检测细胞活力、生长增殖和毒性的四唑盐法(MTT)。四唑盐(MTT)试剂制备说明和应用示例。

Protocol Guide: XTT Assay for Cell Viability and Proliferation

XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

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Global Trade Item Number

SKU	GTIN
R0883-100ML	04061836694036
R0883-6X1L	04061836694067
R0883-6X500ML	04061836694074
R0883-10L	04061837341434
R0883-1L	04061838259943
R0883-20L	04061837341441
R0883-24X500ML	04061836694043
R0883-500ML	04061836694050