R1780
RPMI-1640 Medium
With ʟ-glutamine, sodium bicarbonate without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, suitable for isotope labeling for cell culture applications
Synonym(s):
RPMI 1640 Cell Culture Medium, Roswell Park Memorial Institute 1640 medium
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About This Item
Product Name
RPMI-1640 Medium, With L-glutamine and sodium bicarbonate. Without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling for cell culture applications
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: 2 g/L
phenol red: no
glucose: 2 g/L (Dextro)
L-glutamine: 0.3 g/L
shipped in
ambient
storage temp.
2-8°C
General description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
Preparation Note
Designed for use in stable isotope labeling with amino acids in cell culture applications.
Supplement with 0.100 g/L L-Arginine-13C6, 15N4 HCl (Product 608033), 0.100 g/L L-Leucine-13C6, 15N (Product 608068), and 0.100 g/L L-Lysine-13C6, 15N2 (Product 608041).
Supplement with 0.100 g/L L-Arginine-13C6, 15N4 HCl (Product 608033), 0.100 g/L L-Leucine-13C6, 15N (Product 608068), and 0.100 g/L L-Lysine-13C6, 15N2 (Product 608041).
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
Guokai Yan et al.
Scientific reports, 7(1), 1914-1914 (2017-05-17)
Leucine (Leu) is a multifunctional essential amino acid that plays crucial role in various cellular processes. However, the integral effect of Leu on the hepatic proteome remains largely unknown. Here, we for the first time applied an isobaric tags for
Zhang Xiao Ying et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 22(21), 5383-5393 (2016-11-03)
This study was performed to further our understanding of the biological and genetic basis of follicular lymphoma and to identify potential novel therapy targets. We analyzed previously generated whole exome sequencing data of 23 follicular lymphoma cases and one transformed
Rachel S Lee et al.
Neoplasia (New York, N.Y.), 21(4), 389-400 (2019-03-23)
Approximately 50% of prostate cancers harbor the TMPRSS2:ERG fusion, resulting in elevated expression of the ERG transcription factor. Despite the identification of this subclass of prostate cancers, no personalized therapeutic strategies have achieved clinical implementation. Kinases are attractive therapeutic targets
Global Trade Item Number
| SKU | GTIN |
|---|---|
| R1780-500ML | 04061836694500 |