R3380
Ribonucleoside vanadyl complexes
liquid
Synonym(s):
vanadyl ribonucleoside complexes
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.52
Biological source:
non-animal source
Form:
liquid
Solubility:
H2O: soluble
Storage temp.:
−20°C
Product Name
Ribonucleoside vanadyl complexes, 200 mM
biological source
non-animal source
Quality Level
form
liquid
concentration
200 mM
solubility
H2O: soluble
shipped in
dry ice
storage temp.
−20°C
General description
Ribonucleoside vanadyl complexes are stable complexes formed between ribonucleotides. They are low molecular weight inhibitors of cellular ribonucleases (RNases) that are used for the isolation of RNA from cells.
Application
Ribonucleoside vanadyl complexes are used as ribonuclease inhibitors during cell lysis and mRNA purification, to protect mRNA during cDNA production, and to protect RNA during digestion of DNA by DNaseI.
Ribonucleoside vanadyl complexes has been used:
- as a component of lysis buffer for cell lines prior to Poly(U) pull down assay
- to inhibit nucleases prior to immunoprecipitation (IP) of m6A-containing RNA fragment
- as a component of hybridization buffer for human retinal slices
Biochem/physiol Actions
Ribonucleoside vanadyl complexes (RVC) elicits antimicrobial functionality by inhibiting ribosomal subunit formation and suppressing the growth of Staphylococcus aureus and Escherichia coli. It is also used as a preservative solution for tissues.
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Inhibition of intractable nucleases with ribonucleoside--vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytes.
S L Berger et al.
Biochemistry, 18(23), 5143-5149 (1979-11-13)
Hyone-Myong Eun
Enzymology Primer for Recombinant DNA Technology (1996)
Effect of ribonucleoside-vanadyl complexes on enzyme-catalyzed reactions central to recombinant deoxyribonucleic acid technology.
R S Puskas et al.
Biochemistry, 21(19), 4602-4608 (1982-09-14)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| R3380-5ML | 04061837693908 |