R5886
RPMI-1640 Medium
HEPES modification, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Roswell Park Memorial Institute 1640 medium
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About This Item
Product Name
RPMI-1640 Medium, HEPES Modification, With 25 mM HEPES, without L-glutamine., liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
>7.2
components
L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
General description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good′s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.
Application
RPMI-1640 Medium has been used:
- to culture human lymphoblastoid cell line cells
- prostate cancer cells
- monocytes from human blood
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Stephanie Puukila et al.
International journal of radiation biology, 95(8), 1072-1084 (2019-05-30)
Purpose: Diagnostic radiation is an important part of patient care in the Intensive Care Unit; however, there is little data on the acute effects of exposure to these doses. We investigated pulmonary and splenic response 30 minutes, 4 hours or 24 hours after
Marga J Bouma et al.
Stem cell research, 41, 101654-101654 (2019-11-18)
Lymphoblast cells from four individuals of a family of two genetically unrelated parents and their monozygotic twins were used to generate integration-free human induced pluripotent stem cells (hiPSCs). Reprogramming factors were delivered by co-electroporation of three episomal-based plasmids expressing OCT3/4
Katrin Kleinmanns et al.
EBioMedicine, 56, 102783-102783 (2020-05-27)
The completeness of resection is a key prognostic indicator in patients with ovarian cancer, and the application of tumour-targeted fluorescence image-guided surgery (FIGS) has led to improved detection of peritoneal metastases during cytoreductive surgery. CD24 is highly expressed in ovarian
Global Trade Item Number
| SKU | GTIN |
|---|---|
| R5886-24X500ML | 04061836695750 |
| R5886-100ML | 04061836695743 |
| R5886-500ML | 04061836695767 |
| R5886-6X500ML | 04061838259967 |