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R7509

RPMI-1640 Medium

With sodium bicarbonate, without ʟ-glutamine and phenol red, liquid, sterile-filtered,suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
Technical Service
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Product Name

RPMI-1640 Medium, Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: no
L-glutamine: no
HEPES: no
sodium pyruvate: no
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
RPMI-1640 Medium is suitable for culturing human normal and neoplastic leukocytes. 

Application

RPMI-1640 Medium has been used:
  • to culture Mantle cell lymphoma cells
  • to culture mouse podocytes
  • in the extraction of whole bone marrow from rat Femur and tibia bones

Other Notes

Phenol red has been shown to interfere with the growth of some cells at clonal densities.
Use this medium when working with stem cells or when growing cells at low densities.
Also recommended for in vitro diagnostics use.


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Global Trade Item Number

SKUGTIN
R7509-1L04061836696382
R7509-6X1L04061836696429
R7509-24X500ML04061836696399
R7509-500ML04061836696412
R7509-6X500ML04061836696474