R7638
RPMI-1640 Medium
Dutch Modification, with sodium bicarbonate and HEPES, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Roswell Park Memorial Institute 1640 medium
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About This Item
Product Name
RPMI-1640 Medium, Dutch Modification, with sodium bicarbonate and 20mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
>7.2
components
sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO3: yes
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
General description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
Application
RPMI-1640 Medium has been used
- to obtain spleen and lymph nodes from mice
- for the resuspension of Staphylococcus aureus strains in the phagocytosis assay
- in the isolation of hepatitis B surface antigen-specific B-cell clones
Preparation Note
Supplement with 0.3 g/L L-glutamine.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| R7638-500ML | 04061836696542 |
| R7638-6X500ML | 04061836696573 |