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Merck
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RAB0222

Human IFN γ ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

IFN-gamma, Interferon gamma

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About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... IFNG(3458)
Input:
sample type plasma
sample type urine
sample type serum
sample type cell culture supernatant(s)
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type plasma
sample type urine
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 20.6-15000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IFNG(3458)

General description

The Human IFN- ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IFN- in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human IFN-γ

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human IFN γ ELISA Kit has been used for determining interferon-gamma levels in cell culture supernatant and macrophages.

Biochem/physiol Actions

IFNG (interferon-γ) is a type-2 interferon, which is produced by Th (T helper cells)-1 lymphocytes, CD8 cytotoxic lymphocytes, natural killer (NK) cells, and natural killer T (NKT) cells. It plays an important role in innate and adaptive immune responses, and helps in defense against bacterial infections, dimorphic yeast and some viral infections. High expression of IFNG is common in many autoimmune diseases, such as systemic lupus erythematosis, Sjögren syndrome, inflammatory bowel disease and multiple sclerosis.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.


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Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
    SDS

  • ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids

Regulatory Information

常规特殊物品
低风险生物材料

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Benjamin J Murdock et al.
Journal of immunology (Baltimore, Md. : 1950), 193(8), 4107-4116 (2014-09-17)
The potent immunoregulatory properties of IL-10 can counteract protective immune responses and, thereby, promote persistent infections, as evidenced by studies of cryptococcal lung infection in IL-10-deficient mice. To further investigate how IL-10 impairs fungal clearance, the current study used an
Shuxian Hu et al.
PloS one, 9(8), e105219-e105219 (2014-08-19)
The ability of neural stem/progenitor cells (NSCs) to self-renew, migrate to damaged sites, and differentiate into neurons has renewed interest in using them in therapies for neurodegenerative disorders. Neurological diseases, including viral infections of the brain, are often accompanied by
Vibha Jha et al.
PloS one, 9(8), e104484-e104484 (2014-08-15)
Environmental factors including drugs, mineral oils and heavy metals such as lead, gold and mercury are triggers of autoimmune diseases in animal models or even in occupationally exposed humans. After exposure to subtoxic levels of mercury (Hg), genetically susceptible strains



Global Trade Item Number

SKUGTIN
RAB0222-1KT04061834960744