RAB0874
Porcine INS / Insulin ELISA Kit
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About This Item
NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
Porcine ... INS(397415)
Input:
sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type cell culture supernatant(s)
sample type serum
Species reactivity:
porcine
Storage temp.:
−20°C
Shipped in:
wet ice
species reactivity
porcine
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
input
sample type plasma
sample type cell culture supernatant(s)
sample type serum
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 4 μIU/mL
standard curve range: 4.69 μIU/ml-300 μIU/ml
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
Porcine ... INS(397415)
General description
The antibody reacts specifically against insulin by RIA and immunocytochemistry. It exhibits cross-reactivity with human proinsulin. The antibody binds to insulin with an affinity constant of 8.8 × 109 M-1 in RIA. The insulin gene is mapped to human chromosome 11p15.5.
Human insulin is made up of 51 amino acids. It consists of two peptide chains, 21 amino acids make up A-chain and chain B contains 31 amino acids. The two chains are linked together by a disulfide bond, to form a classical insulin fold with three α helices, chain A having two and one in chain B.
Human insulin is made up of 51 amino acids. It consists of two peptide chains, 21 amino acids make up A-chain and chain B contains 31 amino acids. The two chains are linked together by a disulfide bond, to form a classical insulin fold with three α helices, chain A having two and one in chain B.
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
Abnormal insulin secretion or function, leads to diabetes mellitus, obesity and other carbohydrate metabolism disorders. Insulin is known to stimulate cell division. The biological effects of insulin are mediated by insulin receptor and it integrates major signaling pathways.
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Find documentation for the products that you have recently purchased in the Document Library.
Kinetic properties of monoclonal insulin antibodies.
Keilacker H, et al.
Biomedica Biochimica Acta, 45(8), 1093-1102 (1986)
Proinsulin-Specific Monoclonal Antibodies: Immunocytochemical Application as ?-Cell Markers and as Probes for Conversion.
Madsen O D
Diabetes, 36(10), 1203-1211 (1987)
The molecular mechanism of insulin action.
Kahn C R
Annual Review of Medicine, 36(1), 429-451 (1985)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| RAB0874-1KT | 04061835166466 |