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RDD002

HEPES

≥99.5%, crystalline powder, anhydrous, Redi-Dri™

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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About This Item

Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
7365-45-9
Molecular Weight:
238.30
NACRES:
NA.25
PubChem Substance ID:
329824215
UNSPSC Code:
12161700
EC Number:
230-907-9
MDL number:
MFCD00006158
Beilstein/REAXYS Number:
883043
eCl@ss:
32129211
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Product Name

HEPES, anhydrous, free-flowing, Redi-Dri™, ≥99.5%

grade

anhydrous

Quality Level

100

product line

Redi-Dri™

assay

≥99.5%

form

crystalline powder

quality

free-flowing

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 500 mg/mL, clear, colorless

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
  • In the novel vesicle-budding protocol as a component in stock solution and buffer solutions
  • As a component in intracellular solution for the preparation of brain-slice recording
  • As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification
  • To maintain the pH at 7 in thermal denaturation assay of purified protein
  • In isocratic elution of protein using gel filtration
  • Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

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Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCG1864
SLCD7691
SLCC2031
SLCB6217
SLBZ2837

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Articles

Redi-Dri™ – The World of Free-flowing Hygroscopic Products

Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

Optogenetic Mapping of Synaptic Connections in Mouse Brain Slices to Define the Functional Connectome of Identified Neuronal Populations.
Mingote S, et al.
The Journal of Neuroscience (2015)
Discovery and characterization of a prevalent human gut bacterial enzyme sufficient for the inactivation of a family of plant toxins.
Koppel N, et al.
eLife, 7, e33953-e33953 (2018)
Cell-free Generation of COPII-coated Procollagen I Carriers.
Yuan L, et al.
Bio-protocol, 7(22) (2017)
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Global Trade Item Number

SKUGTIN
RDD002-1KG04061836824679
RDD002-100G04061832550152
RDD002-2.5KG04061836824723