S1629
Sulfatase from Aerobacter aerogenes
Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL
Synonym(s):
Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
2-5 units/mg protein (biuret)
Concentration:
10-20 units/mL
type
Type VI
Quality Level
form
buffered aqueous glycerol solution
specific activity
2-5 units/mg protein (biuret)
mol wt
~41 kDa
concentration
10-20 units/mL
foreign activity
β-Glucuronidase ≤10 U/mL
shipped in
wet ice
storage temp.
−20°C
General description
Sulfatases comprise Cys/Ser-X-Pro-X-Arg motif that is conserved in their active sites.
Application
Sulfatase from Aerobacter aerogenes has been used:
- as a deconjugation enzyme for treating plasma samples for quercetin quantification using liquid chromatography with tandem mass spectrometry (LC/MS/MS) analyses
- in fluorescence intensity-based enzymatic assay with an activity-based probe probe 1
- to treat sulfatide liposomes to remove the 3-O-sulfogalactosyde head from sulfatides
Biochem/physiol Actions
Sulfatases hydrolyze sulfate ester bonds to generate inorganic sulfates. Microbial sulfatases participate in sulfur scavenging and are essential enzymes for the utilization of sulfur. They may be associated with pathogenesis. Commercially available sulfatase from Aerobacter aerogenes is useful as a deconjugation enzyme for the removal of glucuronate and sulfate moieties from conjugates. Sulfatases find application in industry and agriculture.
Physical form
Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37 °C.
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Related Content
Toshiyuki Nakamura et al.
Bioscience, biotechnology, and biochemistry, 75(8), 1506-1510 (2011-08-09)
β-Glucuronidase and sulfatase are the major deconjugating enzymes used in the cleavage of the glucuronate and sulfate moieties, respectively, from certain conjugated food factors including polyphenols. In the present study, we found that compounds having the same molecular weights as
Aphichat Trakooncharoenvit et al.
European journal of nutrition, 59(4), 1389-1398 (2019-05-13)
To investigate the effects of water-soluble dietary fibers (pectin, soybean fiber, and guar gum) on the bioavailability of quercetin glucoside mixture (Q3GM) comprising quercetin-3-O-glucoside (Q3G, 31.8%) and its glucose adducts. Male Wistar/ST rats were fed test diet containing 0.7% Q3GM
Melanie Glauser et al.
Clinica chimica acta; international journal of clinical chemistry, 430, 125-128 (2014-01-15)
Total (i.e. free+sulfated) metanephrines in plasma is a biomarker for the diagnosis of pheochromocytoma/paraganglioma. Sulfated metanephrines must be completely deconjugated by perchloric acid hydrolysis or sulfatase treatment prior to analytical measurement to enable quantification by current techniques. In this report
Global Trade Item Number
| SKU | GTIN |
|---|---|
| S1629-10UN | 04061836911768 |
| S1629-50UN | 04061832759685 |