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S8884

Safranin O

Name: Safranin O
Brand: SIGMA

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A

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About This Item

Empirical Formula (Hill Notation):

C₂₀H₁₉ClN₄

CAS Number:

477-73-6

Molecular Weight:

350.84

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24899820

EC Number:

207-518-8

MDL number:

MFCD00011759

Colour Index Number:

50240

Beilstein/REAXYS Number:

3924099

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Properties

Product Name

Safranin O, certified by the BSC

agency

certified by the BSC

Quality Level

200

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

green to very dark green

solubility

H₂O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

InChI

1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

InChI key

OARRHUQTFTUEOS-UHFFFAOYSA-N

Description

General description

Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.

Application

Safranin O has been used to stain matrix proteoglycans to study cartilaginous tissue structural integrity in mice. It has also been used to stain chondrogenically differentiated human bone marrow stromal cells.

Safranin O has been used in the staining of extraocular muscle mesenchymal stem cells (EOM-MSC) for the detection of cartilage, mucin and mast cell granules It has also been used as a mediator dye in cytochrome P450 spectral assay.

Certified as a stain for chromosomes in the Flemming triple stain; to stain gram negative bacteria in the Gram stain procedure; Safranine-fast green FCF stain for plant cells.

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pictograms

GHS05

signalword

Danger

hcodes

H318

pcodes

P280 - P305 + P351 + P338

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Paul A. Banaszkiewicz

Classic Papers in Orthopaedics (2014)

Measurement of cytochrome P450 and NADPH-cytochrome P450 reductase

Guengerich FP, et al.

Nature Protocols, 4(9), 1245-1245 (2009)

Maria Dannessa Delost

Introduction to Diagnostic Microbiology for the Laboratory Sciences (2014)

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Global Trade Item Number

SKU	GTIN
S8884-100G	04061835574049
S8884-25G	04061836961138