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SAB4200232

Anti-GAD65 Antibody

Name: Anti-GAD65 Antibody
Brand: SIGMA

mouse monoclonal, N-GAD65

Synonym(s):

Anti-GAD2, Anti-glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), Anti-glutamate decarboxylase 65 kDa isoform

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03455043

Conjugate:

unconjugated

Clone:

N-GAD65, monoclonal

Application:

IHC, IP, WB

Citations:

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Properties

Product Name

Anti-GAD65 antibody , Mouse monoclonal, clone N-GAD65, purified from hybridoma cell culture

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

N-GAD65, monoclonal

form

buffered aqueous solution

mol wt

antigen ~65 kDa

species reactivity

human, mouse, monkey, rat

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 0.25-0.5 μg/mL using whole extracts of rat brain

isotype

IgG1

UniProt accession no.

Q05329

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)

Description

General description

Glutamate decarboxylase 2 (GAD2) encodes GAD65, an isoform of glutamate decarboxylase. GAD65 is majorly expressed in axon terminal regions in neurons. GAD65 is present in the synaptic vesicles. GAD2 is mapped to human chromosome 10p12.1. It has an N-terminal pyridoxal 5′-phosphate (PLP) binding −domain.

Application

Monoclonal Anti-GAD65 antibody produced in mouse has been used in the detection of recombinant GAD65 using ELISA.

Biochem/physiol Actions

Glutamate decarboxylase 2 (GAD2) protein GAD65 catalyzes the formation of γ-aminobutyric acid (GABA) from glutamic acid. Palmitoylation of GAD65 is crucial for protein sorting in synaptic clusters. GAD65 autoantigen is prevalent in type 1 diabetes. Its expression increases on allergen exposure. GAD65, along with γ-aminobutyric acid (GABA), may play a key role in the pathogenesis of asthma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品

低风险生物材料

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Certificates of Analysis (COA)

Lot/Batch Number

048M4845

100M4877

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Global Trade Item Number

SKU	GTIN
SAB4200232-200UL	04061837725142