Anti-MAP1LC3B Antibody

The gene MAP1LC3B (microtubule associated protein 1 light chain 3 β) is a homolog of ATG8 gene, an autophagy-related gene in yeast. The encoded protein is called pro-MAP1LC3B, a pro-form that is cleaved by ATG4 to produce a cytosolic form called MAP1LC3B-I. Anti-LC3B antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma LC3B-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the N-terminal region of human LC3B.

synthetic peptide corresponding to the N-terminal region of human LC3B, conjugated to KLH. The corresponding sequence differs by one amino-acid in rat and mouse.

Anti-LC3B antibody produced in rabbit has been used in:

• western blotting
• immunofluorescence
• confocal laser microscopy
• immunoprecipitation

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

MAP1LC3B-I (microtubule associated protein 1 light chain 3 β-I) conjugates with MAP1LC3B-II before being inserted into both inner and outer membranes of the growing autophagic vesicle during autophagy. The cellular distribution and processing of MAP1LC3B facilitates monitoring of autophagy. Cells that lack MAP1LC3B are found to be defective in the formation of autophagic vesicles, with altered phenotypes associated with the role of autophagy in energy homeostasis and removal of protein aggregates. It can serve as an autophagic marker.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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