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SAB4200761

Anti-Vimentin Antibody

Name: Anti-Vimentin Antibody
Brand: SIGMA

mouse monoclonal, LN-6

Synonym(s):

Anti-VIM

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

Clone:

LN-6, monoclonal

Species reactivity:

rat, human, porcine, monkey, bovine, canine, mouse, rabbit, hamster

Application:

FACS, IF, IHC, IP

Citations:

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Properties

Product Name

Anti-Vimentin antibody, Mouse monoclonal, clone LN-6, purified from hybridoma cell culture

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

LN-6, monoclonal

form

buffered aqueous solution

mol wt

~58 kDa

species reactivity

rat, human, porcine, monkey, bovine, canine, mouse, rabbit, hamster

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1 mg/mL

technique(s)

flow cytometry: suitable, immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast Hs68 cell line., immunofluorescence: 1-2 μg/mL using human foreskin fibroblast Hs68 cell line., immunohistochemistry: 5-10 μg/mL using Human breast carcinoma sections., immunoprecipitation (IP): suitable

isotype

IgM

UniProt accession no.

P08670

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

bovine ... Vim(280955)
dog ... Vim(477991)
hamster ... Vim(100689099)
human ... VIM(7431)
mouse ... Vim(22352)
pig ... Vim(100522394)
rabbit ... Vim(100008924)
rat ... Vim(81818)
rhesus monkey ... Vim(705289)

Description

General description

Anti-Vimentin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the LN-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse. Vimentin protein is localized in a wide range of cell types, including pancreatic precursor cells, sertoli cells, mesangial cells, trophoblastic giant cells, neuronal precursor cells, fibroblasts, macrophages, endothelial cells of lung blood vessels, renal tubular cells, neutrophils, leukocytes and renal stromal cells.

Vimentin is a specific type III intermediate filament (IF) protein. It is abundantly expressed in mesenchymal cells. The gene encoding vimentin is located on human chromosome 10p13.

Immunogen

Human thymic nuclear extract

Application

Anti-Vimentin antibody, Mouse monoclonal may be used in:

immunoblotting
immunofluorescence
flow cytometry
immunoprecipitation
immunohistochemistry

Biochem/physiol Actions

Vimentin helps to preserve the structure of cells and stability of tissue in mesenchymal cells. It is involved in tumorigenesis, EMT (epithelial mesenchymal transition) and the metastatic of cancer. It also controls infectious internalization of human papillomavirus 16 pseudovirions.

Anti-Vimentin antibody, Mouse monoclonal specifically recognizes a unique epitope of Vimentin protein which is not expressed in cells of hematopoetic derivation. The antibody is reactive with benign and malignant tumors of mesenchymal and neural derivation but does not react with normal and malignanat human lymphoid cells or derived malignancies. The antibody reacts with Vimentin from human, hamster, mouse, rat, canine, monkey, bovine, porcine and rabbit origin.

Vimentin protein plays a significant role in supporting and anchoring the cellular position of organelles such as the nucleus, endoplasmic reticulum, mitochondria, maintenance of cell shape, the endurance of mechanical stress of mesenchymal cells and motility. Overexpression of the vimentin gene is observed in various epithelial cancers, including prostate, gastrointestinal tumors, central nervous system (CNS) tumors, breast, malignant melanoma and lung cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

Regulatory Information

常规特殊物品

低风险生物材料

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Certificates of Analysis (COA)

Lot/Batch Number

057M4813V

51170306

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Global Trade Item Number

SKU	GTIN
SAB4200761-100UL	04061833265222