SAE0045
HRV-3C Protease.
N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL
Synonym(s):
Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥5000 U/mg
Assay:
≥90% (SDS-PAGE)
Biological source:
human (human Rhinovirus Type 14)
Concentration:
0.8-1.2 mg/mL
biological source
human (human Rhinovirus Type 14)
Quality Level
assay
≥90% (SDS-PAGE)
form
aqueous solution
specific activity
≥5000 U/mg
mol wt
21 kDa
concentration
0.8-1.2 mg/mL
technique(s)
protein purification: suitable
suitability
suitable for protein modification
application(s)
life science and biopharma
shipped in
dry ice
storage temp.
−20°C
General description
HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.
Application
HRV-3C Protease has been used to remove the GST tag from the DsCystatin protein.
Physical form
Supplied in a solution containing 50 mM Tris HCl (pH 7.5), 0.15 M NaCl, 1 mM TCEP and 50% (V/V) glycerol.
Other Notes
One unit of HRV-3C Protease is defined as the amount of enzyme needed to digest 1nmole of the substrate peptide per hour (H-Glu-Ala-Leu-Phe-Gln-pNA) at 0 °C, in a reaction buffer consisting of 25 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.
Still not finding the right product?
Explore all of our products under HRV-3C Protease.
Storage Class
10 - Combustible liquids
wgk
WGK 2
Regulatory Information
常规特殊物品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
Proteases for biotinylated tag removal for protein purification workflows with related reagents and technical resources.
Micah Rapp et al.
Cell reports, 35(1), 108950-108950 (2021-04-02)
Antibodies with heavy chains that derive from the VH1-2 gene constitute some of the most potent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-neutralizing antibodies yet identified. To provide insight into whether these genetic similarities inform common modes of recognition, we
An immunosuppressive tick salivary gland protein DsCystatin interferes with Toll-like receptor signaling by downregulating TRAF6
Ta S et al.9
Frontiers in Immunology, 9 (2018)
Richard W Birkinshaw et al.
Molecular cell, 81(10), 2123-2134 (2021-04-02)
A body of data supports the existence of core (α2-α5) dimers of BAK and BAX in the oligomeric, membrane-perturbing conformation of these essential apoptotic effector molecules. Molecular structures for these dimers have only been captured for truncated constructs encompassing the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SAE0045-1MG | 04061826692509 |