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SRE0007

Mutanolysin from Streptomyces globisporus ATCC 21553

Name: Mutanolysin from <I>Streptomyces globisporus</I> ATCC 21553
Brand: SIGMA

Suitable for manufacturing of diagnostic kits and reagents, ≥4000 units/mg protein (biuret)

Synonym(s):

Mutanolysin

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About This Item

CAS Number:

55466-22-3

UNSPSC Code:

12352204

NACRES:

NA.54

MDL number:

MFCD00131635

Specific activity:

≥4000 units/mg protein (biuret)

Biological source:

bacterial (Streptomyces globisporus)

Concentration:

≥40% protein

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Properties

biological source

bacterial (Streptomyces globisporus)

Quality Level

400

form

lyophilized powder

specific activity

≥4000 units/mg protein (biuret)

mol wt

23 kDa

concentration

≥40% protein

suitability

suitable for cell lysis

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

Description

General description

Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control

Application

Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Biochem/physiol Actions

Mutanolysin cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. It lyses Listeria and other gram positive bacteria such as Lactobacillus and Lactococcus.

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

Physical form

Lyophilized powder containing Ficoll^® and sodium succinate buffer salts

Other Notes

One unit will produce a ΔA₆₀₀of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.