SRE0044
Trypsin inhibitor
lyophilized powder, suitable for mammalian cell culture
Synonym(s):
Trypsin inhibitor from Glycine max (soybean), SBTI
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About This Item
Product Name
Trypsin Inhibitor from Glycine max (soybean), Suitable for manufacturing of diagnostic kits and reagents, suitable for cell culture
biological source
Glycine max (soybean)
Quality Level
description
Glycine Max (Soybean)
form
lyophilized powder
mol wt
20 kDa
storage condition
dry at room temperature
technique(s)
cell culture | mammalian: suitable
color
off-white
solubility
0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL, clear, colorless to very faintly yellow
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
2-8°C
General description
Soybean trypsin inhibitor was first isolated by Kunitz. Several other related inhibitors are also found in soybeans. Trypsin inhibitor from soybeans is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges. Soybean trypsin inhibitor inhibits trypsin, and to a lesser extent chymotrypsin and plasmin.
Application
Trypsin has been used in a study to assess the potential application in animal cell culture of an alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26. Trypsin has also been used in a study to improve the detection of fungi in eosinophilic mucin.
Biochem/physiol Actions
This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.
Features and Benefits
Features:
- Qualified Raw Materials: Products that meet the stringent requirements of the Elevated Assurance Program, ensuring compliance with regulatory standards.
- Comprehensive Documentation: Includes essential documents such as certificates, impurity profiles, and declarations to support audits and risk assessments.
- M-Clarity™ Quality Attributes: Products are classified within the MQ300 quality segment or higher, demonstrating superior quality assurance. M-Clarity™ Program
- Confirmed Process Controls: Established processes that ensure consistency and reliability in manufacturing.
- Enhanced Change Notification: Timely updates on any changes to product specifications or processes, ensuring transparency and compliance.
- Freely Available Dossiers: Access to robust, ready-to-present product dossiers that include:
- Site quality assessment
- ISO 9001 certificate
- Detailed product specifications
- Relevant declarations/statements (e.g., animal origin content, BSE/TSE, nitrosamine)
- Regulatory Compliance: Helps you meet compliance standards effectively, reducing the risk of regulatory issues.
- Audit Readiness: Instills confidence during audits with comprehensive documentation and confirmed process controls, demonstrating your commitment to high-quality products.
- Operational Transparency: Supports supply chain transparency, allowing you to track and verify the quality of raw materials.
- Enhanced Support: Provides exceptional quality-related information and tailored assistance to meet your specific needs.
- Risk Mitigation: Facilitates thorough risk assessments with detailed documentation, helping you manage potential compliance challenges.
- Informed Decision-Making: Access to clear and comprehensive product information enables better decision-making and enhances operational efficiency.
Preparation Note
The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Other Notes
One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.
The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.
Legal Information
M-CLARITY is a trademark of Merck KGaA, Darmstadt, Germany
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26 with potential application in animal cell culture
Manjusha, K., et al.
Cytotechnology (2012)
Canting Guo et al.
Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, 147(5), 943-949 (2012-06-13)
To investigate the improvement in histologic detection of fungi with Gomori methenamine silver (GMS) stain by trypsin predigestion in the mucus of patients with chronic rhinosinusitis (CRS). Prospective, single group, descriptive analysis. Multi-institutional. Thirty-four sinus specimens from 12 surgical patients
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SRE0044-1G | 04061826650196 |
| SRE0044-250MG | 04061833226711 |
| SRE0044-5G | 04061833335031 |