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Merck
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STRN10

Spectrum Plant Total RNA Kit

greener alternative

sufficient for 10 purifications

Synonym(s):

Plant Total RNA Kit

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About This Item

NACRES:
NA.52
UNSPSC Code:
41105501
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usage

sufficient for 10 purifications

Quality Level

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

RNA purification: suitable

test parameters

: ~30 min kit run time, sample volume: 100 mg

greener alternative category

storage temp.

15-25°C

General description

The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.

Features and Benefits

  • Yields up to 60 μg of pure concentrated RNA per prep
  • Efficient protocol allows for RNA purification in 30 min or less
  • Specially designed for research with difficult plant tissues

Legal Information

Spectrum is a trademark of Sigma-Aldrich Co. LLC


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Kit Components Only

Product No.
Description

  • Lysis Solution

  • Binding Solution

  • Wash Solution 1

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • 2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
    SDS

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

target_organs

Liver,Heart

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

154.4 °F

flash_point_c

68 °C

wgk

WGK 3

Regulatory Information

危险化学品

This item has



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Related Content

The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.


Qun Cheng et al.
Frontiers in plant science, 11, 272-272 (2020-04-08)
Soybean [Glycine max (L.) Merr.] is an important crop for oil and protein resources worldwide, and its farming is impacted by increasing soil salinity levels. In Arabidopsis the gene EARLY FLOWERING 3 (ELF3), increased salt tolerance by suppressing salt stress
De novo Assembly of Leaf Transcriptome in the Medicinal Plant Andrographis paniculata.
Cherukupalli N
Frontiers in Plant Science, 7, 1203-1203 (2016)
Genomic approaches for interrogating the biochemistry of medicinal plant species.
Gongora-Castillo E
Methods in Enzymology, 517, 139-159 (2012)



Global Trade Item Number

SKUGTIN
STRN10-1KT04061835574995