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STRN50

Spectrum Plant Total RNA Kit

greener alternative

sufficient for 50 purifications

Synonym(s):

Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit

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About This Item

NACRES:
NA.52
UNSPSC Code:
41105501
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usage

sufficient for 50 purifications

Quality Level

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

RNA purification: suitable

test parameters

: ~30 min kit run time, sample volume: 100 mg

greener alternative category

storage temp.

15-25°C

General description

The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.

Application

Spectrum Plant Total RNA Kit has been used to isolate RNA from ground fiber tissue of cultured cotton fiber, from neem tissues and from Sarracenia, an insectivorous plant.

Features and Benefits

  • Yields up to 60 μg of pure concentrated RNA per prep
  • Efficient protocol allows for RNA purification in 30 min or less
  • Specially designed for research with difficult plant tissues

Legal Information

Spectrum is a trademark of Sigma-Aldrich Co. LLC


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Kit Components Only

Product No.
Description

  • Binding Solution

  • Lysis Solution

  • Wash Solution 1

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • 2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
    SDS

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

target_organs

Liver,Heart

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

Regulatory Information

危险化学品

This item has



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Articles

简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA,包括哺乳动物、植物和细菌培养物来源的细胞和组织。

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Related Content

The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.


Transcriptome analysis of sarracenia, an insectivorous plant.
Srivastava A
DNA Research : An International Journal for Rapid Publication of Reports on Genes and Genomes, 18(4), 253-261 (2011)
Soybean DICER-LIKE2 Regulates Seed Coat Color via Production of Primary 22-Nucleotide Small Interfering RNAs from Long Inverted Repeats.
Jia, et al.
Plant Cell, 32, 3662-3673 (2021)
Gil Wiseglass et al.
Scientific reports, 9(1), 6476-6476 (2019-04-26)
Obligate root holoparasite Phelipanche aegyptiaca is an agricultural pest, which infests its hosts and feeds on the sap, subsequently damaging crop yield and quality. Its notoriously viable seed bank may serve as an ideal pest control target. The phytohormone abscisic



Global Trade Item Number

SKUGTIN
STRN50-1KT04061835575008