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T1270

Thiamine hydrochloride

Name: Thiamine hydrochloride
Brand: SIGMA

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

Synonym(s):

Aneurine hydrochloride, Vitamin B₁ hydrochloride

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About This Item

Empirical Formula (Hill Notation):

C₁₂H₁₇ClN₄OS · HCl

CAS Number:

67-03-8

Molecular Weight:

337.27

NACRES:

NA.75

PubChem Substance ID:

24899952

eCl@ss:

34058006

UNSPSC Code:

12352207

EC Number:

200-641-8

MDL number:

MFCD00012780

Beilstein/REAXYS Number:

3851771

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Properties

biological source

synthetic (organic)

Quality Level

200

product line

BioReagent

assay

≥99.0% (HPLC)

form

powder

mol wt

M_w 337.27 g/mol

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

color

white

mp

250 °C (dec.) (lit.)

solubility

H₂O: 50 mg/mL, clear, colorless

application(s)

agriculture

SMILES string

CC1=NC(N)=C(C[N+]2=CSC(CCO)=C2C)C=N1.Cl.[Cl-]

InChI

1S/C12H17N4OS.2ClH/c1-8-11(3-4-17)18-7-16(8)6-10-5-14-9(2)15-12(10)13;;/h5,7,17H,3-4,6H2,1-2H3,(H2,13,14,15);2*1H/q+1;;/p-1

InChI key

DPJRMOMPQZCRJU-UHFFFAOYSA-M

Description

General description

Thiamine or Vitamin B1 is a positively charged and essential constituent for all tissues. It is present at higher levels in skeletal muscles, liver, kidney, heart, and brain. Thiamine is composed of a pyrimidine ring linked to a thiazole ring via a methylene bridge.

Application

Thiamine hydrochloride has been used as a component:

of the vitamin-concentrated stock solution for the preparation of Hv-Ca medium for culturing Haloferax volcanii
of the tris-acetate-phosphate (TAP) medium for culturing Chlamydomonas nivalis
of modified Bold 3N medium for culturing Chlorella minutissima

Biochem/physiol Actions

Thiamine is converted to thiamine diphosphate post-movement into the cellular membranes by thiamine diphosphokinase. Thiamine diphosphate, an active cofactor is essential for the mechanism of many enzymes involved in the citric acid cycle, the glycolytic pathway, the degradation of branched-chain amino acids, and the pentose phosphate pathway. Thiamine is involved in converting food into energy required for functioning the central and peripheral nervous systems. Lower levels of this vitamin are associated with delirium, Wernicke′s encephalopathy, and Wernicke-Korsakoff syndrome. Thiamine hydrochloride is required to support energy metabolism and amino acid synthesis in cultured cells. It is present in many classical and serum-free formulations.

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pictograms

GHS07

signalword

Warning

hcodes

H319

pcodes

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Articles

Thiamine in Cell Culture

Explore thiamine's impact on serum-free cell culture systems for biomanufacturing monoclonal antibodies.

Development of A Fission Yeast Cell-Based Platform for High Throughput Screening of HIV-1 Protease Inhibitors.

Zsigmond Benko et al.

Current HIV research, 17(6), 429-440 (2019-11-30)

HIV-1 protease inhibitor (PI) is one of the most potent classes of drugs in combinational antiretroviral therapies (cART). When a PI is used in combination with other anti- HIV drugs, cART can often suppress HIV-1 below detection thus prolonging the

Gene expression differences in infected and noninfected middle ear complementary DNA libraries.

Joseph E Kerschner et al.

Archives of otolaryngology--head & neck surgery, 135(1), 33-39 (2009-01-21)

To investigate genetic differences in middle ear mucosa (MEM) with nontypeable Haemophilus influenzae (NTHi) infection. Genetic upregulation and downregulation occurs in MEM during otitis media (OM) pathogenesis. A comprehensive assessment of these genetic differences using the techniques of complementary DNA

Multi-Omics Driven Metabolic Network Reconstruction and Analysis of Lignocellulosic Carbon Utilization in Rhodosporidium toruloides.

Joonhoon Kim et al.

Frontiers in bioengineering and biotechnology, 8, 612832-612832 (2021-02-16)

An oleaginous yeast Rhodosporidium toruloides is a promising host for converting lignocellulosic biomass to bioproducts and biofuels. In this work, we performed multi-omics analysis of lignocellulosic carbon utilization in R. toruloides and reconstructed the genome-scale metabolic network of R. toruloides.

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Global Trade Item Number

SKU	GTIN
T1270-100G	04061835569533
T1270-25G	04061835569540