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Merck
CN

T2194

Trizma® hydrochloride solution

pH 7.4, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

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About This Item

NACRES:
NA.25
UNSPSC Code:
12161700
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Quality Level

grade

BioPerformance Certified, Molecular Biology

form

solution

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

pH

7.4

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

General description

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma®hydrochloride solution has been used:
  • in the preparation of cell-lysis buffer
  • as a component of wash buffers and RNase mix 1, used in the preparation of 5′-complete cDNAs for paired-end sequencing
  • in dissolving cadmium or S2- loaded microreactors for CdS synthesis

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany


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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter



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RAMPAGE: Promoter Activity Profiling by Paired-End Sequencing of 5?-Complete cDNAs
Batut P and Gingeras TR
Current Protocols in Molecular Biology, 104(1), 25B-211 (2013)
Determination of Rate of [3H-methyl]-choline Incorporation into Cellular Lipids and Non-lipid Metabolites
Smith T and Phyu S
Bio-protocol, 6(22) (2016)
Lab-on-a-micromotor: catalytic Janus particles as mobile microreactors for tailored synthesis of nanoparticles
Pacheco M, et al.
Chemical Science, 9(42), 8056-8064 (2018)



Global Trade Item Number

SKUGTIN
T2194-100ML04061837341632
T2194-1L04061837341649