Skip to Content
Merck
CN

Key Documents

View All Documentation

T2569

Trizma® hydrochloride solution

pH 7.8, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

NACRES:
NA.25
UNSPSC Code:
12161700
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

200

grade

BioPerformance Certified, Molecular Biology

form

solution

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)

pH

7.8

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

General description

Tris(hydroxymethyl)aminomethane (Tris) is a routine buffer in biological sciences. The pH buffering range is between 7.0 -9.0. It displays negligible binding towards metal in solutions. Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies. Its low ionic mobility finds usage in capillary electrochromatography as well.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma® hydrochloride solution has been used:
  • as a component of lysis buffer for lysing human embryonic kidney (HEK) 293 cells
  • in the 10X digoxigenin (DIG) deoxynucleoside triphosphate (dNTP) nick translation labeling buffer and in tris EDTA and sodium chloride (TEN) buffer
  • in the preparation of tris, acetate, ethylenediaminetetraacetic acid (EDTA) (1x TAE) buffer and as a component of 10X gel loading dye

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Still not finding the right product?

Explore all of our products under Trizma® hydrochloride solution

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCK8253
SLCJ3745
SLCF5993
SLCD2337
SLBX6642

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Global Trade Item Number

SKUGTIN
T2569-100ML04061837342387
T2569-1L04061837342417