Key Documents

View All Documentation

T2885

3,3′,5,5′-Tetramethylbenzidine

Name: 3,3&#8242;,5,5&#8242;-Tetramethylbenzidine
Brand: SIGMA

TMB substrate, chromogenic, ≥98% (TLC), powder

Synonym(s):

BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate

Select a Size

Change View

About This Item

Linear Formula:

[-C₆H₂(CH₃)₂-4-NH₂]₂

CAS Number:

54827-17-7

Molecular Weight:

240.34

NACRES:

NA.83

PubChem Substance ID:

24900090

UNSPSC Code:

12352204

EC Number:

259-364-6

MDL number:

MFCD00007748

Beilstein/REAXYS Number:

2808541

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

3,3′,5,5′-Tetramethylbenzidine, ≥98% (TLC)

Quality Level

200

assay

≥98% (TLC)

form

powder

mp

168-171 °C (lit.)

solubility

ethyl acetate: 50 mg/mL, clear to very slightly hazy

storage temp.

2-8°C

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

Description

Application

3,3′,5,5′-Tetramethylbenzidine has been used:

as a supplement in Man-Rodosa-Sharpe (MRS) agar plate, to stains the lactobacilli colonies blue in the presence of O2
as a substrate solution for ELISA (enzyme-linked immunosorbent assay)
in myeloperoxidase activity assay to quantify neutrophil

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Biochem/physiol Actions

3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in in situ free available chlorine (FAC) monitoring of chlorite-based sanitizers.