T3069
Trizma® hydrochloride solution
pH 8.0, BioPerformance Certified, 2 M, suitable for cell culture
Synonym(s):
Tris hydrochloride solution
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
Quality Level
grade
BioPerformance Certified, Molecular Biology
form
solution
concentration
2 M
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm Heavy metals (as Pb)
color
colorless
pH
7.95—8.05, 8.0
useful pH range
7.0-9.0
density
1.07—1.09 g/cm3
absorption
≤0.05 at 290 at 40%
General description
Tris(hydroxymethyl)aminomethane (Tris) has a pH buffering range between 7.0 - 9.0 and is a routine buffer in biological sciences. Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies. It displays negligible binding towards metal in solutions.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.
Application
Trizma® hydrochloride solution has been used as a component of lysis buffer for testing on commercially available biological sample preservation paper types. It has also been used as a vehicle and for dissolving leptin in reporter gene assay studies.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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Elke Ericson et al.
Molecular and cellular endocrinology, 412, 257-264 (2015-05-20)
In a world with increasing incidences of obesity, it becomes critical to understand the detailed regulation of appetite. To identify novel regulators of the signaling mediated by one of the key hormones of energy homeostasis, leptin, we screened a set
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T3069-100ML | 04061838175762 |
| T3069-1L | 04061838260215 |