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T3073

Tissue Preparation Solution

30 mL sufficient for 1,000 extractions

Synonym(s):

Direct PCR tissue preparation solution, Extract-N-Amp™ Tissue Preparation Solution, REDExtract-N-Amp™ Tissue Preparation Solution, SYBR® Green Extract-N-Amp™ Tissue Preparation Solution

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About This Item

UNSPSC Code:
12352200
EC Number:
254-457-8
NACRES:
NA.55
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Quality Level

200

usage

30 mL sufficient for 1,000 extractions, sufficient for 1000 reactions

feature

Direct PCR

technique(s)

PCR: suitable

input

crude DNA

shipped in

wet ice

storage temp.

−20°C

General description

The Tissue Preparation Solution is intended for use with the Extract-N-Amp™ Tissue PCR kits, REDExtract-N-Amp™ Tissue PCR kits, and SYBR® Green Extract-N-Amp Tissue PCR kits. This product is intended to supplement the kit reagents, where there are many extractions.

Application

Tissue preparation solution has been used:
  • in combining segments of tails for genotyping
  • to extract genomic DNA from zebrafish embryos/fin tissues
  • to extract DNA from ear tags, tail tips and embryonic yolk sacs of mouse embryo
  • for extraction of DNA from fungal spores and mycelia

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCH7607
SLCH7606
SLCH0131
SLCH0146
079M4055V

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Protocols

REDExtract-N-Amp™组织PCR试剂盒实验方案

exrract-N-Amp试剂盒实验方案提供了一种快速的DNA提取方法，可在15分钟内获得PCR样品。优化的PCR ReadyMix能成功产生清洁的扩增。RED上样染料实现样品跟踪。

REDExtract-N-Amp™ Tissue PCR Kit Protocol

Extract-N-Amp kit protocol provides rapid DNA extraction yielding PCR-ready samples in 15 minutes with optimized PCR ReadyMix.

SYBR® Green Extract-N-Amp™ PCR Kit

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

N-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironment.
Soh BS
Cell Research, 24(12), 1420-1432 (2014)
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
Varshney GK, et al.
Nature Protocols, 11(12), 2357-2357 (2016)
Jessica Wallingford et al.
Frontiers in molecular neuroscience, 10, 268-268 (2017-09-14)
Astrocyte dysfunction has been indicated in many neurodevelopmental disorders, including Fragile X Syndrome (FXS). FXS is caused by a deficiency in fragile X mental retardation protein (FMRP). FMRP regulates the translation of numerous mRNAs and its loss disturbs the composition

Global Trade Item Number

SKUGTIN
T3073-.3ML04061838250131
T3073-30ML04061833768112
T3073-3ML04061838101266