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T5326

Anti-γ-Tubulin Antibody

Name: Anti-γ-Tubulin Antibody
Brand: SIGMA

mouse monoclonal, GTU-88

Synonym(s):

Anti-Tubulin Antibody

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00677366

Clone:

GTU-88, monoclonal

Species reactivity:

rat, hamster, chicken, human, bovine, canine, Xenopus, mouse

Application:

ARR, ELISA (i), ICC, IP, WB

Citations:

266

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Properties

Product Name

Anti-γ-Tubulin antibody, Mouse monoclonal, clone GTU-88, purified from hybridoma cell culture

biological source

mouse

Quality Level

200

antibody form

purified antibody (purified from hybridoma cell culture)

antibody product type

primary antibodies

clone

GTU-88, monoclonal

form

buffered aqueous solution

mol wt

antigen 48 kDa

species reactivity

rat, hamster, chicken, human, bovine, canine, Xenopus, mouse

packaging

antibody small pack of 25 μL

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1 mg/mL

technique(s)

immunocytochemistry: 1-2 μg/mL using HeLa cells, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using total cell extract of chicken fibroblasts

isotype

IgG1

UniProt accession no.

P23258

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBG1(7283)
mouse ... Tubg1(103733)
rat ... Tubg1(252921)

Description

General description

γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ-Tubulin consists of two isoforms tubulin γ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ-tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.

Immunogen

synthetic γ-tubulin peptide, conjugated to KLH

Application

Monoclonal Anti-γ-Tubulin antibody produced in mouse has been used in following studies:

Western blot analysis
Immunohistochemistry
Immunocytology

Biochem/physiol Actions

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression of γ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

常规特殊物品

低风险生物材料

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Global Trade Item Number

SKU	GTIN
T5326-25UL	04061838511102
T5326-100UL	04061837347337
T5326-200UL	04061837347344