T5569
3,3′,5,5′-Tetramethylbenzidine
peroxidase substrate, chromogenic, liquid
Synonym(s):
TMB substrate for ELISA (Super Slow)
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
Product Name
3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA, peroxidase substrate
form
liquid
shipped in
wet ice
storage temp.
2-8°C
General description
3,3′,5,5′-Tetramethylbenzidine (TMB) is an electrochemical substrate for low level detection of horse radish peroxidase (HRP). It is a highly sensitive and dual function substrate, since it produces both soluble and insoluble reaction products. It has rapid reaction rate and it is suitable for on-line kinetic analysis. It does not possess carcinogenic properties.
Application
3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA has been used as a substrate for enzyme linked immunosorbent assay (ELISA).
Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 60% slower than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Soluble amyloid-beta buffering by plaques in Alzheimer disease dementia versus high-pathology controls
Esparza TJ, et al.
PLoS ONE, 13(7), e0200251-e0200251 (2018)
ApoE4 accelerates early seeding of amyloid pathology
Liu CC, et al.
Neuron, 96(5), 1024-1032 (2017)
Combinatorial Chemistry, 114-114 (1996)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T5569-100ML | 04061837347962 |
| T5569-4X100ML | 04061837347986 |