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T6146

Trypan Blue

Name: Trypan Blue
Brand: SIGMA

powder, BioReagent, suitable for cell culture

Synonym(s):

Direct blue 14, Niagara blue reagent, blue live/dead cell assay

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About This Item

Linear Formula:

C₃₄H₂₄N₆O₁₄S₄Na₄

CAS Number:

72-57-1

Molecular Weight:

960.81

EC Number:

200-786-7

UNSPSC Code:

12352207

NACRES:

NA.75

PubChem Substance ID:

57654686

MDL number:

MFCD00003969

Beilstein/REAXYS Number:

4360496

Colour Index Number:

23850

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Properties

product line

BioReagent

Quality Level

300

form

powder

composition

Dye content, ~40%

packaging

glass bottle of 100 g, glass bottle of 25 g, glass bottle of 5 g

storage condition

dry at room temperature

technique(s)

cell culture | mammalian: suitable, tissue processing: suitable

mp

>300 °C (lit.)

solubility

H₂O: 10 mg/mL

application(s)

cell analysis

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

Gene Information

human ... PTPN1(5770), PTPRF(5792)

Description

General description

The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.

Application

Trypan blue has been used:

in cell viability assay
to stain plant tissue in order to to visualize cell death

Biochem/physiol Actions

The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.

Preparation Note

To make a cell viability solution, add the T6146 Trypan Blue powder to a clean and dry glass or plastic container of appropriate capacity. Add a suitable solvent, such as sterile distilled water, to the container with the Trypan Blue powder. Stir the solution vigorously using a stir bar or a glass rod until the powder has completely dissolved. A 0.4% solution is a common concentration for typical cell viability assessment. For example, to make 100 mL of a 0.4% solution, add 400 milligrams (0.4 g) to 99.6 mL solvent.

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pictograms

GHS08

signalword

Danger

hcodes

H350

pcodes

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Global Trade Item Number

SKU	GTIN
T6146-100G	04061835569632
T6146-25G	04061835569649
T6146-5G	04061835569656