T6258
Triosephosphate Isomerase from rabbit muscle
Type X, lyophilized powder, ≥3,500 units/mg protein
Synonym(s):
D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-774-2
MDL number:
Specific activity:
≥3,500 units/mg protein
type
Type X
Quality Level
form
lyophilized powder
specific activity
≥3,500 units/mg protein
mol wt
calculated mol wt 53.2 kDa
composition
Protein, 60-90% biuret
foreign activity
pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
storage temp.
−20°C
General description
Triosephosphate isomerase (TPI) gene is located on human chromosome 12p13. It is expressed in all tissues. This homodimer of two 27 kDa subunits is highly stable and is made up of 248 amino acids. TPI is catalytically active in its dimeric state.
Application
Triosephosphate Isomerase from rabbit muscle has been complexed with p-hydroxymercurybenzoate (PHMB) to denature the protein for hydrophobic interaction chromatography coupled online with chemical vapour atomic fluorescence spectrometry (HIC−CVGAFS).
Triosephosphate isomerase has been used in a study to assess changes in cardiac energy metabolic pathways in overweight rats fed a high-fat diet. Triosephosphate isomerase has also been used in a study to investigate proteomic identification of carbonylated proteins in F344 rat hippocampus after 1-bromopropane exposure.
Biochem/physiol Actions
Triosephosphate isomerase (TPI) is responsible for the catalysis of the triose phosphate isomers, dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. TPI deficiency is associated with glycolytic enzymopathy. Lack of TPI is also associated with chronic hemolytic anemia, enhanced exposure to bacterial infections, cardiomyopathy and neuromuscular disease.
Packaging
Package size based on protein content
Physical form
Sulfate-free, contains EDTA and borate buffer salts
Other Notes
One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
低风险生物材料
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Related Content
Triosephosphate isomerase deficiency: new insights into an enigmatic disease
Orosz F, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1792(12), 1168-1174 (2009)
Hydrophobic interaction chromatography coupled with atomic fluorescence spectrometric detection: Effect of the denaturation on the determination of thiolic proteins
Bramanti E, et al.
Talanta, 63(2), 383-389 (2004)
Red blood cell enzymopathies
Hematology, 616-625 (2018)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T6258-1MG | 04061837358517 |
| T6258-5MG | 04061833274682 |