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T7951

Tau Protein Ladder, 6 isoforms human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

Tau Protein Ladder

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD09265460
Form:
buffered aqueous glycerol solution
Assay:
≥90% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli
Mol wt:
36800, 39700, 40000, 42600, 42900, 45900
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biological source

human

Quality Level

200

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

36800, 39700, 40000, 42600, 42900, 45900

composition

dodecyl sulphate sodium salt, 1-5% , glycerine, 20-30% , mercaptoethanol, 10-20%

technique(s)

immunoelectrophoresis: 10-20 μL using recombinant Tau protein marker, western blot: 2-5 μL using recombinant Tau protein marker

UniProt accession no.

P10636

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MAPT(4137)

General description

Research area: Neuroscience

Tau gene, spanning 100 kb with 16 exons, is mapped to human chromosome 17q21. The six alternative splice variants of protein ranging in size from 352-441 amino acids have been identified in human adult brain. Tau is a member of the microtubule-associated protein (MAP) family. It is predominantly expressed in neurons.

Application

Tau Protein Ladder, 6 isoforms human has been used as a sample in immunomagnetic reduction (IMR) assay. It has also been used as positive control in western blotting.

Biochem/physiol Actions

In neurons, tau protein plays an important role in maintaining microtubule assembly and stability. It acts as a connector between microtubules and other cytoskeletal elements or proteins. Overexpression of the protein has been observed in Alzheimer′s disease and various neurodegenerative disorders denoted as ‘tauopathies′. Thus, pathological tau proteins act as a potential biomarker in the neurodegenerative process.

Features and Benefits

Contains 6 recombinant Tau proteins expressed in E. coli, which comprise six Tau isoforms with molecular weights of 45,900, 42,600, 42,900, 39,700, 40,000, and 36,800 respectively. No histidine-tags are present in the proteins.

Packaging

A 50 μL vial of Tau Protein Ladder contains 0.25 μg of each of the six isoforms.

Physical form

Solution in 125 mM Tris-HCl, pH 6.8, containing 4% SDS, 10% 2-mercaptoethanol, 20% glycerol, and 0.004% bromphenol blue.

Preparation Note

Tightly closed. Keep in a well-ventilated place. Keep locked up or in an area accessible only
to qualified or authorized persons

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

target_organs

Liver,Heart

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK8565
SLCH1921
SLCC2608
SLCB2444
SLBX3807

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Reeteka Sud et al.
Molecular therapy. Nucleic acids, 3, e180-e180 (2014-07-30)
In Alzheimer's disease, progressive supranuclear palsy, and a number of other neurodegenerative diseases, the microtubule associated protein tau aggregates to form intracellular neurofibrillary tangles and glial tangles, abnormal structures that are part of disease pathogenesis. Disorders with aggregated tau are
Analytical performance of reagent for assaying tau protein in human plasma and feasibility study screening neurodegenerative diseases
Yang S, et al.
Scientific reports, 7(1), 9304-9304 (2017)
Tau protein in cerebrospinal fluid: a biochemical marker for axonal degeneration in Alzheimer disease?
Blennow K, et al.
Molecular and Chemical Neuropathology, 26(3), 231-245 (1995)
View All Related Papers

Global Trade Item Number

SKUGTIN
T7951-50UL04061837378782