TOX4
In Vitro Toxicology Assay Kit, Neutral Red based
Synonym(s):
NR uptake assay, Neutral red assay
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About This Item
NACRES:
NA.84
UNSPSC Code:
12352207
form
liquid
Quality Level
usage
1 kit sufficient for 1,000 tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
λmax
540 nm
application(s)
cell analysis
detection
detection method
colorimetric
storage temp.
2-8°C
General description
For spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 540 nm.
Application
In Vitro Toxicology Assay Kit, Neutral Red based has been used:
- to measure the lysosomal enzyme activity in HB8902 cells
- to study the cell viability of human alveolar epithelial cells
- to study the cell viability in fibroblasts
Biochem/physiol Actions
Neutral red (NR) is a red dye that accumulates in the lysosome by actively transferring across the cell membranes. The dye is extracted and the uptake is quantitated by spectroscopy. The NR uptake assay is the most commonly used cytotoxicity test. It is widely applicable in environmental and biomedical studies to estimate the number of viable cells in culture. The NR dye in vitro tissue culture studies is used in assessing viral cytopathogenicity and detection of toxic compounds.
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B - Flam. Liq. 3 - Skin Sens. 1
Storage Class
3 - Flammable liquids
wgk
WGK 3
flash_point_f
73.4 °F
flash_point_c
23 °C
Regulatory Information
危险化学品
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Related Content
Barbara Kazuń et al.
BMC veterinary research, 16(1), 216-216 (2020-06-27)
The aim of the study has been to compare the effect of dietary supplementation of β-1,3/1,6-glucan, Lactobacillus plantarum bacteria or their mixture on the growth performance, selected parameters of the immune system as well as the liver and intestinal histology
R Fautz et al.
Mutation research, 253(2), 173-179 (1991-10-01)
The neutral red (NR) absorption method was adapted for the determination of cell viability in the UDS assay with primary hepatocyte cultures of the rat. The NR method is rapid, easy to perform, and suitable for handling of large numbers
E Borenfreund et al.
Toxicology letters, 24(2-3), 119-124 (1985-02-01)
A method is described which combines the use of a visual morphological cytotoxicity assay with a quantitative neutral red (NR) spectrophotometric test, for the assessment of the effect of toxic agents on 3T3 cells in culture. These sensitive and reproducible
Global Trade Item Number
| SKU | GTIN |
|---|---|
| APREST70390-100UL | 04061835935901 |
| APREST70391-100UL | 04061835935918 |
| HPA017880-25UL | 04061842836284 |
| HPA017880-100UL | 04061836323264 |
| HPA027551-100UL | 04061835658381 |
| HPA027551-25UL | 04061842856824 |
| TOX4-1KT | 04061837397943 |
| EMU200211-20UG | 04061828772612 |
| EMU200211-50UG | 04061828720378 |