TOX6
In Vitro Toxicology Assay Kit, Sulforhodamine B based
Synonym(s):
biomass viability assay, sulforhodamine B, total biomass assay
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About This Item
NACRES:
NA.84
UNSPSC Code:
12352207
form
liquid
Quality Level
usage
kit sufficient for 1,000 tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
λmax
565 nm
application(s)
cell analysis
detection
detection method
colorimetric
storage temp.
room temp
General description
The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.
Application
In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein. It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines. Absorbance of dye is measured at a wavelength of 565nm.
Biochem/physiol Actions
Dye binds to cellular protein and is then solubilized in base.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
Storage Class
8A - Combustible corrosive hazardous materials
wgk
WGK 2
Regulatory Information
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Articles
基于细胞的细胞增殖（BrdU、MTT、WST1）、细胞活力和细胞毒性实验，可应用于癌症、神经科学和干细胞研究。
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
Related Content
Immune expression and inhibition of heat shock protein 90 in uveal melanoma
Faingold D, et al.
Clinical Cancer Research, 14(3), 847-855 (2008)
Monocarboxylate transporters (MCTs) in gliomas: expression and exploitation as therapeutic targets
Miranda G, et al.
Neuro-Oncology, 15(2), 172-188 (2012)
Evaluation of colorimetric protein and biomass stains for assaying drug effects upon human tumor cell lines.
Skehan, P., et al.
Proceedings of the American Association For Cancer Research, 30, 2436-2436 (1989)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| TOX6-1KT | 04061837397950 |