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TRUE9

TrueGel3D Hydrogel Kits

SLO-PVA, PEG non cell-degradable crosslinker

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About This Item

NACRES:
NA.71
UNSPSC Code:
12352207
Storage temp.:
−70°C
Shipped in:
dry ice
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shipped in

dry ice

storage temp.

−70°C

General description

The gel is formed by stable thioether bonds between thiol groups on PEG non cell-degradable crosslinker and thio-reactive groups on SLO-PVA. The polymer, SLO-PVA is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-PVA. SLO-PVA does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.

Application

TRUE9-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic in-vivo conditions and to investigate the effect of matrix stiffness on cell physiology and behavior.SLO-PVA based gels are used to grow cells that do not need attachment to hydrogel for survival but rather use their secreted extracellular matrix. The SLO-PVA based hydrogels are preferred over dextran-based gels for long-term culture of cells producing carbohydrate degrading enzymes. TrueGel3DTM Hydrogel Kits has been used in the generation of 3D gel based tumor models.

Features and Benefits

  • Slow gelling (takes 6 minutes to 25 minutes, depends on gel stiffness)
  • Flexibility in modulating gel stiffness
  • Does not allow cell migration
  • Does not allow cell recovery and post-culture analyses
  • Cell inert hydrogel (if not customized with RGD peptide)

Other Notes

TRUE9-1KT contains SLO-PVA solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer and water.

Disclaimer

Avoid prolong exposure of PEG non cell-degradable crosslinker to air and store SLO-PVA in small portions to avoid frequent freeze-thaw cycles.

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pictograms

Corrosion
GHS05

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

wgk

WGK 3

Storage Class

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
0001622521
0001615167
0006165167

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Protocols

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

TrueGel3D™ Hydrogel Prep Protocol

TrueGel3D™ is a chemically defined hydrogel for 3D cell growth, facilitating microchannel filling with slow gelation.

Articles

3D细胞培养水凝胶

水凝胶是最常用的 3D 细胞培养系统。了解关于该技术的更多信息（什么是水凝胶？如何使用水凝胶？）

3D细胞培养水凝胶_细胞培养_TrueGel3D™水凝胶-默克生命科学

TrueGel3D™细胞培养水凝胶是一种由混合聚合物与交联剂混合而成的生化成分限定的水凝胶。与其他基于动物细胞生物提取物（例如小鼠EHS肿瘤细胞）的水凝胶相比，TrueGel3D™细胞培养水凝胶不含任何可能会干扰或造成实验污染的动物源成分。

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

View All Articles

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Explore our portfolio of 3D cell culture tools and technologies for any 3D cell line or application and discover organoids, hydrogels, ULA, microwell, or hydrogel plates, and more.

Product Information Sheet - TRUE9
Design of Spherically Structured 3D In vitro Tumor Models-Advances and Prospects
Ferreira LP, et al.
Acta Biomaterialia (2018)

Global Trade Item Number

SKUGTIN
TRUE9-1KT04061835539321